It contain contain only one organism. Pure culture is always rooted in an integrated culture (multifaceted) by transferring a small sample into a new, basic growth in such a way that it disperses individual cells in a central location or by multiplying the sample multiple times before adding a new sample. Both methods separate the individual cells so that, as they multiply, each will form an inactive colony, which can then be used to enter the middle mass, with the assurance that only one species will exist. The separation of pure culture can be improved by offering a mixed and medium inoculate that promotes the growth of one organ in the involvement of others.
WHAT IS BIOREMEDIATION?
Treating dirt or debris (such as oil, dirty groundwater, or industrial processes) by using microorganisms (such as bacteria) that breaks down unwanted substances.Bioremediation may b in a (local) and ex- situ (removal and treatment elsewhere).
Pollutants: Soil pollution presents various health risks. Soil pollution is often classified as an organic and inorganic pollution.
- Biological contamination can be pests and pests.
- The inorganic impurities can be arsenic, copper, cadmium.
Organic pollutants: Sr. no Class of pollutant Example More potential origin 1 BTEX Benzene Methyl benzene Oil extraction Gas plant 2 Fungicides 1, 3, 5- triazine Generating station\nInorganic pollutants: Pollutant Source Arsenic Manure Cadmium Dye, stain Bioremediation agents: Natural materials, whether native or foreign, are important agents used in bioremediation. Organic substances vary, depending on the chemical properties of the pollutants, and should be selected with caution as they only support a specified limit for chemical pollutants.
The first patent for biodegradation was recorded in 1974, becoming a Pseudomonas putida strain that is able to reduce fuel. Bioremediation can occur naturally or through interventions. Bacteria: Bacterial are very different organisms, and thus make the best players involved in biodegradation and bioremediation. There is very little universal poisoning that kills bacteria, so it is possible that something can break down any surface, provided it is provided with the right conditions (anaerobic versus aerobic environment, sufficient electron donors or acceptors, etc.).
Below are some known bacteria that are involved in bioremediation:
- Pseudomonas putida: Pseudomonas putida is a nasty soil bacterium involved in the bioremediation of toluene, which is part of a small amount of paint. It can also destroy naphthalene, a by-product of petroleum refining, in contaminated soils.
- Dechloromonas aromatic: Dechloromonas aromatica is a rod-shaped bacterium that can increase aromatics including benzoate, chlorobenzoate, and toluene, combining the reaction with a decrease in oxygen, chlorate, or nitrate.
It is the only organism capable of adding benzene anaerobically. Due to the high concentrations of benzene pollutants, especially in water and underwater, D. aromatic is particularly useful for the bioremediation of this substance.
Nitrifiers and De- nitrifiers:
Industrial bioremediation is used to purify wastewater. Most treatment programs rely on microbial activity to remove unwanted nitrogen compounds (e.g. Ammonia, nitrite, nitrate). Nitrogen removal is a two-phase process involving nitrization and denitrification. During nitrification, ammonium is added to nitrite by substances such as Nitrosomonas europaea.Then, nitrite is also transported nitrate by bacteria such as Nitrobacter hamburgensis.
In anaerobic conditions, nitrate produced during ammonium oxidation is used as a lethal electronic receptor by pathogens such as Paracoccus denitrificans. The result is N2 gas. Through this process, ammonium and nitrate, the pollutants responsible for eutrophication in natural water, are recycled.
Deinococus radiodurans: Deinococcus radiodurans are a bacterium resistant to genetically engineered radiation for bioremediation of sol and heavy metals. Engineering type Deinococcus radiodurans has been shown to degrade ionic mercury and toluene in mixed waste dumps.
In anaerobic conditions, nitrate produced during ammonium oxidation is used as a lethal electronic receptor by pathogens such as Paracoccus denitrificans. The result is dinitrogen gas. Through this process, ammonium and nitrate, the pollutants responsible for eutrophication in natural water, are recycled.
Methylibium petroleiphilum: Methylibium petroleiphilum (officially known as PM1 type) is a bacterium capable of causing methyl tetra-butyl ether (MTBE) bioremediation. PM1 degrades MTBE by using pollutants as the sole source of carbon and energy.
Alcanivorax borkumensis: Alcanivorax borkumensis is a bacterium that is shaped by seaweed that feeds on hydrocarbons, such as those found in gasoline, and produces carbon dioxide. It grows rapidly in oil-damaged areas, and has been used to help refine more than 830,000 liters of oil from the Deepwater Horizon oil spill in the Gulf of Mexico. Fungi (mycoremediation): Current applications of bioremediation mainly use bacteria, with few attempts to use mold. Fungi have important fundamental roles because of their role in biomass and the regeneration of organisms and organisms. These features can be translated into bioremediation applications that can break down organic computers and reduce the risk of metals.
- Fungi have an advantage over bacteria not only in their metabolic flexibility but also in their natural stability.
- They are able to emit a number of different chemicals and survive in harsh environmental conditions such as low humidity and high concentrations of pollutants. Therefore, fungi are the most powerful tools in soil bioremediation and other adaptive species such as White Rot Fungi.
Biodegradation capacities of white rot fungi: Fungus is used as a potential treatment for pollution that began in 1985 when the white decaying species of Phanerochaete chrysosporium were found to remedy significant environmental pollutants.
- White rot mold has a great advantage because it degrades lignin extracellularly through its hyphal expansion.
- This allows them to access soil pollutants that other organisms cannot afford to maximize the surface area of the enzymatic interaction.
- These inexpensive molds can withstand extreme environmental conditions, such as pH, temperature, and moisture content.
- Phanerochaete chrysosporium: The first chrysosporium fungus connected to biological contamination, and pollution.
Further studies have shown this to have great potential for bioremediation in pesticides, PAHs, dioxins, carbon tetra chlorides, and many other pollutants. The power of white mold molds, especially models of the phanerochaete chrysoporium model to destroy various pollutants. However, xenobiotic contamination rarely looks at the problem with wood, natural habitat is white mold. The details of the delivery of wood for bioremediation cotton have been adopted from mushroom growers, who have perfected the art of producing lignocellulosic waste. Factors affecting microbial remediation:
- Soil structure,
- Solubility in water,
- Chemical structure.
Advantages of bioremediation:
- Bioremediation is a natural process..
- Toxic chemicals are destroyed or removed locally and not just isolated.
- Lower expenditure.
- Less power is needed as compared to other technologies
- Handmade steering.
- Limited to those including the Malformed. Not all compounds can get into this rapid and complete destruction
- It is difficult to get out of balance on a bench and on a pilot scale to complete full field measurements.
- Requires a very capable person
- Often take longer than other treatments, such as digging and removing soil or burning.